il10 blocking antibody (R&D Systems)
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il10 blocking antibody
Il10 Blocking Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il10+blocking+antibody/Rat+IgG1+Isotype+Control/pmc07189341-103-71-74
Average 95 stars, based on 132 article reviews
Il10 Blocking Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 132 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/il10+blocking+antibody/Rat+IgG1+Isotype+Control/pmc07189341-103-71-74
Average 95 stars, based on 132 article reviews
il10 blocking antibody - by Bioz Stars,
2026-10
95/100 stars
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1) Product Images from "IL10 restrains autoreactive B cells in transgenic mice expressing inactive RAG1"
Article Title: IL10 restrains autoreactive B cells in transgenic mice expressing inactive RAG1
Journal: Cellular immunology
doi: 10.1016/j.cellimm.2018.06.004
Figure Legend Snippet: (A). Splenocytes from WT or dnRAG1 mice were cultured for 72h with or without LPS (10 μg/mL) in the absence or presence of the following: recombinant IL10 (rIL10), IL10 blocking antibody (α-IL10 Ab) or isotype control (Iso Ab), or ruxolitinib (Rux), SH-4–54, triamcinolone acetonide (TA) or vehicle (DMSO), and plasma cell subsets were analyzed by flow cytometry as in Fig. 2B. Summary data for n=3 animals/genotype are presented in bar graph format and represented as mean +/− SEM. Statistically significant differences using the Tukey correction are indicated (a, vs LPS; b, vs LPS+Iso Ab; c, vs LPS+DMSO). (B) Concentrations of IgM and IgG in supernatants from splenocyte cultures shown in panel A were measured by ELISA.
Techniques Used: Cell Culture, Recombinant, Blocking Assay, Control, Flow Cytometry, Enzyme-linked Immunosorbent Assay
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Cell culture Single-cell suspensions from spleen were adjusted to 4.0×10 6 cells/mL in sterile RF10M and 2.0×10 5 cells dispensed into wells of a sterile 96-well flat bottom plate (Corning #353072) The cells were mixed with an equal volume of RF10M lacking or containing the following reagents (source and final concentration shown in parentheses): LPS (Sigma, L2880, 10 μg/mL), Concentration Assay:Article Title: IL10 restrains autoreactive B cells in transgenic mice expressing inactive RAG1 Article Snippet: FloJo software was used for data analysis (TreeStar, Ashland, OR). .. 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Single-cell suspensions from spleen were adjusted to 4.0×10 6 cells/mL in sterile RF10M and 2.0×10 5 cells dispensed into wells of a sterile 96-well flat bottom plate (Corning #353072) The cells were mixed with an equal volume of RF10M lacking or containing the following reagents (source and final concentration shown in parentheses): LPS (Sigma, L2880, 10 μg/mL), Article Title: IL10 restrains autoreactive B cells in transgenic mice expressing inactive RAG1. Article Snippet: FloJo software was used for data analysis (TreeStar, Ashland, OR). .. 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